abstract：:The human growth hormone/human chorionic somatomammotropin (hGH/hCS) gene cluster contains five genes: hGH-N, hGH-V, hCS-B, and hCS-L. In this study, the nature of splicing products of their primary transcripts (except hCH-L) was analyzed by nuclease mapping as well as by reverse transcription-polymerase chain reactio...

journal_title：Molecular and cellular endocrinology

authors： Palmetshofer A,Zechner D,Luger TA,Barta A

更新日期：1995-09-22 00:00:00

abstract：:Isolated islets were either studied immediately after isolation (fresh; F), or were cultured for 6 days at 11 mM glucose (desensitized; D), or were incubated for 2 h at 5.5 mM glucose following D (recovered; R). Glucose-stimulated insulin secretion in D islets was reduced compared with F and R islets. In the presence ...

journal_title：Molecular and cellular endocrinology

authors： Laychock SG

更新日期：1995-08-30 00:00:00

abstract：:The pineal hormone melatonin modulates constitutive protein secretion from melanoma M2R cells. Nanomolar concentrations of melatonin inhibited protein secretion early after plating or at low cell density, but facilitated it late after plating or at high cell density. Inhibition by melatonin of adenylate cyclase is the...

journal_title：Molecular and cellular endocrinology

authors： Bubis M,Zisapel N

更新日期：1995-08-11 00:00:00

abstract：:The LHRH receptor in alpha T3-1 gonadotrope cells was shown to bring about a marked and sustained activation of MAP kinase. This response was prevented by protein kinase C inhibition or down-regulation and could be partially mimicked by phorbol ester. Additional evidence for inhibition of this response by pertussis to...

journal_title：Molecular and cellular endocrinology

authors： Sim PJ,Wolbers WB,Mitchell R

更新日期：1995-08-11 00:00:00

abstract：:Desethylamiodarone (DEA), the major metabolite of the potent antiarrythmic drug amiodarone, is a non-competitive inhibitor of the binding of thyroid hormone (T3) to the beta 1-thyroid hormone receptor (T3R). In the present study, we investigated whether DEA acts in a similar way with respect to the alpha 1-T3R. The ch...

journal_title：Molecular and cellular endocrinology

authors： van Beeren HC,Bakker O,Wiersinga WM

更新日期：1995-07-01 00:00:00

abstract：:We examined the effect of growth hormone on local growth factor mRNA expression in male Sprague-Dawley rats. Repetitive systemic administration of growth hormone (0.4 IU every 4 h) increased the expression of IGF-I mRNA up to 2.8-fold in costal cartilage tissue compared with controls. Basic FGF (bFGF) mRNA expression ...

journal_title：Molecular and cellular endocrinology

authors： Izumi T,Shida J,Jingushi S,Hotokebuchi T,Sugioka Y

更新日期：1995-07-01 00:00:00

abstract：:We review in this article recent findings on the effects of cell proximity (nearness to each other) on anterior pituitary cell function in vitro. Important cellular processes, including protein synthesis, basal hormone release, response to hypothalamic regulatory factors and signal transduction can change as a functio...

journal_title：Molecular and cellular endocrinology

authors： Perez FM,Rose JC,Schwartz J

更新日期：1995-06-01 00:00:00

abstract：:The mechanism by which growth hormone-binding protein (GH-BP) is generated in humans remains unclear. To address this question, we analysed human GH-receptor/GH-BP gene expression in a human hepatoma cell line (HuH7). Northern hybridisation showed that HuH7 cells contain a single mRNA species hybridising with a probe ...

journal_title：Molecular and cellular endocrinology

authors： Mullis PE,Holl RW,Lund T,Eblé A,Brickell PM

更新日期：1995-06-01 00:00:00

abstract：:The expression of prostatic acid phosphatase (PAcP) in three human prostate carcinoma cell lines including LNCaP, DU 145 and PC-3, was studied to explore its potential role as a marker in the progression of prostate cancer. Although Southern blot analysis suggested the presence of PAcP gene in all three prostate carci...

journal_title：Molecular and cellular endocrinology

authors： Garcia-Arenas R,Lin FF,Lin D,Jin LP,Shih CC,Chang C,Lin MF

更新日期：1995-04-28 00:00:00

abstract：:Effects of human retinoid X receptor alpha (hRXR alpha) and its ligand, 9-cis-retinoic acid, on T3-mediated auto-regulation of hTR beta 1 gene expression were examined using a chloramphenicol acetyltransferase (CAT) reporter system, and a deletional analysis of the promoter. hRXR alpha enhanced T3-dependent CAT induct...

journal_title：Molecular and cellular endocrinology

authors： Sakurai A,Suzuki S,Katai M,Miyamoto T,Kobayashi H,Nakajima K,Ichikawa K,DeGroot LJ,Hashizume K

更新日期：1995-04-28 00:00:00

abstract：:Human breast cancer cells selected for multidrug resistance frequently overexpress ligands and receptors in the epidermal growth factor (EGF) receptor family. To determine whether this overexpression contributes to the drug resistant phenotype, EGF receptor transfected ZR75B human breast cancer cells were examined. Tw...

journal_title：Molecular and cellular endocrinology

authors： Dickstein BM,Wosikowski K,Bates SE

更新日期：1995-04-28 00:00:00

abstract：:The effect of a gonadotropin-releasing hormone-agonist (GnRH-a) on the synthesis of insulin-like growth factor-binding protein-5 (IGFBP-5), a physiological marker for atresia, was investigated. Granulosa cells obtained from diethylstilbestrol (DES)-treated immature female rats were cultured in serum-free medium for 72...

journal_title：Molecular and cellular endocrinology

authors： Onoda N,Li D,Mickey G,Erickson G,Shimasaki S

更新日期：1995-04-28 00:00:00

abstract：:Insulin and cAMP stimulate prolactin gene transcription and prolactin-CAT expression in rat pituitary tumor GH cells. Expression of prolactin-CAT construct, pPrl(-173/+75)CAT, is stimulated 10- to 30-fold by either insulin or cAMP. Addition of both insulin and cAMP resulted in an additive 20- to 60-fold stimulation. A...

journal_title：Molecular and cellular endocrinology

authors： Jacob KK,Stanley FM

更新日期：1995-04-01 00:00:00

abstract：:In order to define the possible involvement of proteoglycans (PG) in the regulation of Sertoli cell functions, we have examined the effect of para-nitrophenyl-beta-D-xyloside (PNPX), a specific inhibitor of PG synthesis, on follicle stimulating hormone (FSH)-dependent estradiol production by immature rat Sertoli cells...

journal_title：Molecular and cellular endocrinology

authors： Phamantu NT,Bonnamy PJ,Bouakka M,Bocquet J

更新日期：1995-03-01 00:00:00

abstract：:Blocking K+ channels induces hormone secretion in various pituitary cell lines by a mechanism which is not completely delineated. In the present study, we employed the mouse pituitary tumor-derived AtT-20 cell as a model to evaluate this phenomenon. We correlated the effect of the K+ channel-blocker, tetraethylammoniu...

journal_title：Molecular and cellular endocrinology

authors： Wang X,Greer MA

更新日期：1995-03-01 00:00:00

abstract：:The involvement of continuous protein synthesis in the mechanisms of crustacean steroidogenesis was investigated using crayfish molting glands (Y-organs). During intermolt, Y-organ steroidogenic activity is low. Eyestalk ablation initiates premolt which is characterized by a rapid increase in the production of ecdyste...

journal_title：Molecular and cellular endocrinology

authors： Dauphin-Villemant C,Böcking D,Sedlmeier D

更新日期：1995-03-01 00:00:00

abstract：:Transcriptional control of steroid-regulated gene networks by nuclear receptor proteins results in the coordinate expression of a limited number of target genes. Although much is known about the structure and function of steroid receptors, relatively few cell-specific steroid-regulated genes have been isolated and cha...

journal_title：Molecular and cellular endocrinology

authors： Chapman MS,Qu N,Pascoe S,Chen WX,Apostol C,Gordon D,Miesfeld RL

更新日期：1995-02-27 00:00:00

abstract：:Factors that regulate the tissue specific and developmental expression of the GLUT4 gene, whose transcribed protein is primarily responsible for mediating insulin stimulated glucose transport, are poorly defined. In this study we examined the effects of retinoic acid, a circulating factor that can promote cellular dif...

journal_title：Molecular and cellular endocrinology

authors： Sleeman MW,Zhou H,Rogers S,Ng KW,Best JD

更新日期：1995-02-27 00:00:00

abstract：:The endometrial expression of the gene encoding porcine uteroferrin (UF), during pregnancy is presumed to be mediated by cis-regulatory regions distinct from those that confer its limited expression to other mammalian tissues and cell types. In the present study, chimeric DNA constructs of native and progressive 5' de...

journal_title：Molecular and cellular endocrinology

authors： Gonzalez BY,Perera OP,Michel FJ,Simmen RC

更新日期：1995-02-27 00:00:00

abstract：:Bone marrow-derived leukocytes are present in human endometrium/decidua and are believed to serve a variety of functions in this tissue. The number and type of leukocytes in endometrium/decidua vary with the hormonal milieu of the ovarian cycle, with blastocyst implantation, and during pregnancy. The factors that regu...

journal_title：Molecular and cellular endocrinology

authors： Arici A,MacDonald PC,Casey ML

更新日期：1995-02-01 00:00:00

abstract：:To identify cis-acting prolactin (PRL) response elements within the sheep beta-lactoglobulin (BLG) promoter, CHO cells were co-transfected with a rabbit PRL-receptor (PRL-R) expression plasmid and a number of BLG-CAT constructs. Resection through the 4200 bp BLG promoter diminished the PRL response. Mutation of the pr...

journal_title：Molecular and cellular endocrinology

authors： Demmer J,Burdon TG,Djiane J,Watson CJ,Clark AJ

更新日期：1995-01-01 00:00:00

abstract：:The transforming growth factor beta (TGF-beta) superfamily includes several closely related peptides including the activins and inhibins. Since we recently reported that TGF-beta 1 and beta 2 are potent inducers of steroid 5 alpha-reductase (5 alpha R), we have now studied the effects of these other peptides using pri...

journal_title：Molecular and cellular endocrinology

authors： Antonipillai I,Wahe M,Yamamoto J,Horton R

更新日期：1995-01-01 00:00:00

abstract：:Corticotropin (ACTH) regulates glucocorticoid production through specific receptors on the adrenal cortex. Analysis of the ACTH receptor mRNA in human adrenal has revealed the presence of five transcripts ranging from 1.8 to 11 kilobases (kb). Characterization of the 5'-untranslated regions (UTRs) of the ACTH receptor...

journal_title：Molecular and cellular endocrinology

authors： Naville D,Barjhoux L,Jaillard C,Lebrethon MC,Saez JM,Bégeot M

更新日期：1994-12-01 00:00:00

abstract：:Effects of insulin-like growth factor I on the expression of the immediate-early gene c-fos in adrenomedullary tissue were assessed in bovine chromaffin cells in primary culture. The expression of c-fos mRNA and FOS protein were studied by Northern blot and immunofluorescence microscopy. IGF-I induced c-fos mRNA expre...

journal_title：Molecular and cellular endocrinology

authors： Skar R,Larsen TH,Serck-Hanssen G

更新日期：1994-12-01 00:00:00

abstract：:Previous studies have shown that disruption or damage to the seminiferous tubules by radiation, antiandrogen, vitamin A deficiency or experimental cryptorchidism causes Leydig cell hypertrophy and hyperplasia, suggesting that Sertoli cells secrete a mitogenic factor(s) that stimulates Leydig cell proliferation. To stu...

journal_title：Molecular and cellular endocrinology

authors： Wu N,Murono EP

更新日期：1994-12-01 00:00:00

abstract：:The cDNA encoding the bovine activin type II receptor has been cloned by reverse transcription-polymerase chain reaction (RT-PCR) amplification of a bovine testicular RNA preparation. Sequence comparisons of the bovine activin type II receptor with its human, mouse and rat homologues show strong evolutionary conservat...

journal_title：Molecular and cellular endocrinology

authors： Ethier JF,Houde A,Lussier JG,Silversides DW

更新日期：1994-12-01 00:00:00

abstract：:A comparative study of hGH and IL2 post-signaling effects, as examined by RNA expression (Nb29) and protein levels of the heat-shock protein hsp70, was performed in a hormone-dependent rat lymphoma cell line, Nb2-11C. Optimal doses of hGH or IL2 increased Nb29 expression in a dose-dependent manner. Addition of both mi...

journal_title：Molecular and cellular endocrinology

authors： Horn S,Cohen R,Gertler A

更新日期：1994-11-01 00:00:00

abstract：:The peculiarities of cellular and tissue distribution of prolactin receptors (PRLR) in the liver of female and male rats with different sex steroid status were investigated in paraplast-embedded tissue with the indirect immunoperoxidase technique. Two clones of antibodies directed outside the PRL-binding site (U6) or ...

journal_title：Molecular and cellular endocrinology

authors： Smirnova OV,Petraschuk OM,Kelly PA

更新日期：1994-10-01 00:00:00

abstract：:Progesterone elicits a rapid, transient calcium influx in sperm that is a prerequisite for the progesterone-induced acrosome reaction. The possibility that the GABAA receptor/chloride channel was the receptor that mediated the progesterone-induced calcium influx in human sperm was examined. A-ring reduced 3 alpha-hydr...

journal_title：Molecular and cellular endocrinology

authors： Blackmore PF,Im WB,Bleasdale JE

更新日期：1994-09-01 00:00:00

abstract：:17 beta-Hydroxysteroid dehydrogenase type 1 (17-HSD type 1) is a steroidogenic enzyme catalyzing reversible interconversion of estradiol and estrone. 17-HSD type 1 is actively expressed in human placenta. We characterized 17-HSD type 1 expression and its regulation by basic fibroblast growth factor (bFGF) in JAR, JEG-...

journal_title：Molecular and cellular endocrinology

authors： Lewintre EJ,Orava M,Peltoketo H,Vihko R

更新日期：1994-08-01 00:00:00

abstract：:Conventional in vitro autoradiographical techniques have been used to screen a number of ovine peripheral tissues for the presence of specific 2-[125I]iodomelatonin binding sites. Intense specific labelling (defined as that displaced by 1 microM melatonin) was observed in the adrenal cortex, and less intense specific ...

journal_title：Molecular and cellular endocrinology

authors： Helliwell RJ,Howell HE,Lawson W,Barrett P,Morgan PJ

更新日期：1994-08-01 00:00:00

abstract：:In man, two isoforms of growth hormone receptor (GHR) have been reported. The first, hGHR corresponds to the original form described by Leung et al. (J. Biol. Chem. 264 (1987) 9905-9910). The second, hGHRd3, missing 22 N-terminal amino acids is encoded by an mRNA derived from alternative splicing and deletion of the t...

journal_title：Molecular and cellular endocrinology

authors： Esposito N,Paterlini P,Kelly PA,Postel-Vinay MC,Finidori J

更新日期：1994-07-01 00:00:00

abstract：:We studied the ontogeny of GH receptor mRNA levels and the effect of exogenous estradiol administration on GH receptor mRNA levels in rabbit liver. A solution hybridization-RNase protection assay revealed a predominant 370-base long protected band corresponding to the mRNA encoding the transmembrane GH receptor, and a...

journal_title：Molecular and cellular endocrinology

authors： Domené HM,Marín G,Sztein J,Yu YM,Baron J,Cassorla FG

更新日期：1994-07-01 00:00:00

abstract：:The aim of this study was to investigate whether short-term treatment with nitric oxide donors could mimic cytokine inhibition of insulin secretion. We tested the nitric oxide generating compounds 3-morpholinosydnonimine (SIN-1), S-nitroso-N-penicillamine (SNAP), S-nitrosoglutathione and hydroxylamine for their abilit...

journal_title：Molecular and cellular endocrinology

authors： Cunningham JM,Mabley JG,Delaney CA,Green IC

更新日期：1994-06-01 00:00:00

abstract：:Androgen receptor mRNA was translated in vitro, and androgen- and antiandrogen-bound receptor complexes were studied using limited proteolytic digestion by trypsin. Partial proteolysis of androgen-bound receptor protein resulted in a 29-kDa proteolysis-resisting fragment, whereas antiandrogen binding stabilised a 35-k...

journal_title：Molecular and cellular endocrinology

authors： Kuil CW,Mulder E

更新日期：1994-06-01 00:00:00

abstract：:Insulin-like growth factor-I (IGF-I) is involved in the growth and development of liver and brain during fetal life by acting through specific plasma membrane receptors. In an attempt to determine how the changes in IGF-I receptor number are regulated during development, we have compared [125I]IGF-I binding to membran...

journal_title：Molecular and cellular endocrinology

authors： Santos A,Yusta B,Fernández-Moreno MD,Blázquez E

更新日期：1994-05-01 00:00:00

abstract：:The interaction between corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) is important in the regulation of adrenocorticotropin (ACTH) release from the anterior pituitary (AP). CRF exerts its effect on the AP by activating the adenylate cyclase (AC) complex whereas AVP increases the turnover of phosp...

journal_title：Molecular and cellular endocrinology

authors： Liu JP,Robinson PJ,Funder JW,Engler D

更新日期：1994-05-01 00:00:00

abstract：:125I-glucagon was directly cross-linked to its receptor in isolated adipocyte plasma membranes using a UV irradiation procedure. This investigation resulted in identification of an adipocyte glucagon receptor complex of 62 kDa, present both in white and brown adipose tissues. The specificity of labeling was shown by i...

journal_title：Molecular and cellular endocrinology

authors： Iwanij V,Amos TM,Billington CJ

更新日期：1994-05-01 00:00:00

abstract：:Antisense oligonucleotides can inhibit gene expression in living cells by binding to complementary sequences of DNA, RNA or mRNA. The mechanisms include inhibition of RNA synthesis, RNA splicing, mRNA export, binding of initiation factors, assembly of ribosome subunits and of sliding of the ribosome along the mRNA cod...

journal_title：Molecular and cellular endocrinology

authors： Baertschi AJ

更新日期：1994-05-01 00:00:00

abstract：:Using as a model system, primary cultures of porcine Leydig cells, we have shown that transforming growth factor-beta 1 (TGF-beta 1) (2 ng/ml, 72 h) antagonizes the stimulatory action of insulin-like growth factor-I (IGF-I) on luteinizing hormone (LH/hCG) receptors. We therefore investigated the action of TGF-beta 1 o...

journal_title：Molecular and cellular endocrinology

authors： Besset V,Collette J,Chauvin MA,Franchimont P,Benahmed M

更新日期：1994-03-01 00:00:00